Macf1 Knockout cell line (MC3T3-E1)

Macf1 Knockout cell line (MC3T3-E1) Order

Catalog#

YKO-M236

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Macf1 Knockout cell line（MC3T3-E1 Subclone 14）

Cell Morphology

Fibroblast-like, adherent

Passage ratio

1:3~1:5

Species

Mouse

Gene Name

Macf1

Gene ID

11426

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% Alpha MEM+10%FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MC3T3-E1 Subclone 14				STR Info (Cell bank) Cell Line:MC3T3-E1 Subclone 14
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
18-3	15.0				15.0
4-2	20.3				20.3
6-7	17.0				17.0
19-2	13.0				13.0
1-2	19.0				19.0
7-1	26.2				26.2
8-1	16.0				16.0
1-1	16.0	17.0			16.0	17.0
3-2	14.0				14.0
2-1	15.0	16.0			16.0
15-3	19.2	22.3			22.3
6-4	18.0				18.0
13-1	16.1				16.1
11-2	16.0				16.0
17-2	16.0				16.0
12-1	17.0				17.0
5-5	17.0				17.0
X-1	28.0				28.0
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Macf1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC3T3-E1 Subclone 14 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Macf1 gene knockout Macf1 Knockout cell line（MC3T3-E1 Subclone 14） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Macf1 Knockout cell line (MC3T3-E1)

Catalog#

YKO-M236

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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